| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.044470 |
| Chromosome: | chromosome 8 |
| Location: | 1519947 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g364950 | CSE2,CSE1,FAP317 | Coiled-Coil Flagellar Associated Protein 317 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTACAACGACGCCAGCTTCCGCAGCTACCGCAAGCCGCGCAGCCCTC |
| Internal bar code: | TCGATTTAGCATAGTACGAGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4265 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 12 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTGGTGCTCCTTACATGCCA |
| Suggested primer 2: | TGGCTGGCTTCTCTGCTAAC |