Insertion junction: CLIP2.044476_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.044476
Chromosome:chromosome 9
Location:6311353
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g414000 VTE8,HPT1 (1 of 1) 2.5.1.116 - Homogentisate geranylgeranyltransferase / HGGT; homogentisate phytyltransferase/ prenyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCACTGGAACCAGCCCGCGGAGCATGCACCATTCGGCCAGTAGCGCTT
Internal bar code:CGGGTTCACGCTTCAGCGTCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:868
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:ACGTGCTCTGAACCTCCATG
Suggested primer 2:AGTAATCGCTAGCCCGAACG