| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.044483 |
| Chromosome: | chromosome 1 |
| Location: | 4292584 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g029100 | (1 of 1) K10733 - GINS complex subunit 2 (GINS2, PSF2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGACAACATGCTTGCGTCTGTGGTCGCAGAACGACAAGGAGTGGCAGGA |
| Internal bar code: | GTTGCTGGGTGCACAAACGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1470 |
| LEAP-Seq percent confirming: | 81.25 |
| LEAP-Seq n confirming: | 13 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTTAGCTATCAGGGGCTGG |
| Suggested primer 2: | GGCAGCTGGTGTCTTCTTCT |