Insertion junction: CLIP2.044497_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.044497
Chromosome:chromosome 12
Location:2670319
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g505350 FAP10,ACA2 Flagellar Associated Protein 10; (1 of 2) PTHR24093:SF254 - PLASMA MEMBRANE CALCIUM-TRANSPORTING ATPASE 2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTAACTTAGCATTAGCAATCCATACAAGTATCTTTCAGGGCTTGGACAT
Internal bar code:TTGCACGGAGTGAGTCCTTTCC

Confirmation - LEAP-Seq

LEAP-Seq distance:4787
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:63
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGCTTACGGACACAACA
Suggested primer 2:GGGGGCCAGACGTTTTAGAA