| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044511 |
| Chromosome: | chromosome 3 |
| Location: | 661181 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g145667 | (1 of 1) PTHR13060//PTHR13060:SF0 - SGT1 PROTEIN HSGT1 SUPPRESSOR OF GCR2 // PROTEIN SGT1 | intron | |
| Cre03.g145687 | RAD2 | (1 of 1) K04799 - flap endonuclease-1 (FEN1, RAD2); DNA repair protein, XPGC/Rad2 homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCAAATGGAGCAGGGTCTGCCAATGCAGCCTGCCCGTGTTGATGTGCA |
| Internal bar code: | GGGTTTGACAAGCATGGTGCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2835 |
| LEAP-Seq percent confirming: | 94.1176 |
| LEAP-Seq n confirming: | 32 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACCCTACCGTGCATAGGC |
| Suggested primer 2: | TATGCCAGAGTCGTCATCGC |