Insertion junction: CLIP2.044529_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.044529
Chromosome:chromosome 12
Location:7014045
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g560650 (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAGGTGGTCTGGTCGGCACGCACCGGCGCCTCCAGCAGCCCCTCCAGGAT
Internal bar code:GTTCCCCCGTGGTGGCTCTATG

Confirmation - LEAP-Seq

LEAP-Seq distance:1400
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:21
LEAP-Seq n unique pos:42

Suggested primers for confirmation by PCR

Suggested primer 1:GCAACACTGGTGCGTCAAAT
Suggested primer 2:CAAAACCGGGCGAACAATGT