Insertion junction: CLIP2.044542_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.044542
Chromosome:chromosome 2
Location:5295841
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g112850 COQ6 (1 of 1) K06126 - ubiquinone biosynthesis monooxygenase Coq6 (COQ6); Flavin-dependent monoxygenase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCATTCCGCAGCGGCCCCACCGAGTTGATCAGCTCCAAACCCACACCGC
Internal bar code:TACTCGCGAACATCAACGCGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:4034
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:127
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:127

Suggested primers for confirmation by PCR

Suggested primer 1:ATCAGTTCACCCGTTCGCAT
Suggested primer 2:GCTCGGGGACTTGATACTCG