| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.044544 |
| Chromosome: | chromosome 6 |
| Location: | 1253376 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g258700 | PYC1 | (1 of 1) 6.4.1.1 - Pyruvate carboxylase / Pyruvic carboxylase; Pyruvate carboxylase 1 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACTTGAGGTTGGTGTACTGGCCGCCCGGCATCTCGTGCACGTACACGTCA |
| Internal bar code: | CTGTCGTGGTTTAATATCAGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 212 |
| LEAP-Seq percent confirming: | 17.6471 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACAATCGTTGGGAGGCTG |
| Suggested primer 2: | TGTAGCACATCAAGGCTGGG |