Insertion junction: CLIP2.044549_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.044549
Chromosome:chromosome 7
Location:2283156
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g327750 TRP3 Transient receptor potential ion channel protein; (1 of 6) PTHR31942//PTHR31942:SF1 - FAMILY NOT NAMED // MLO-LIKE PROTEIN 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATAGAAGACGGGAAGAGGCGGTAGAGAGCTCGGTGAGCACCGGTAGGAGG
Internal bar code:ACAGTCCAAACACCGATGGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2042
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:85
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:85

Suggested primers for confirmation by PCR

Suggested primer 1:CATACAATTCACCTGCCGCG
Suggested primer 2:GAATCTAGCAACAGCGCGTG