| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044567 |
| Chromosome: | chromosome 12 |
| Location: | 7497822 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g556750 | TIC32,TIC32-1,SDR22 | Short-chain dehydrogenase/reductase; (1 of 2) 1.1.1.330 - Very-long-chain 3-oxoacyl-CoA reductase / Very-long-chain beta-ketoacyl-CoA reductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTTTACGTGGCGTGCCGATTCGTACCGTGCAGTTCGAATCCTAGATAC |
| Internal bar code: | CATAACTCAGACACATTTTAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 931 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCTAACACCAGCAGCTGT |
| Suggested primer 2: | CGGGTGCAGACATTACCAGT |