Insertion junction: CLIP2.044597_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.044597
Chromosome:chromosome 16
Location:7358494
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g679445 FLA1,FAS6 (1 of 10) PTHR10900 - PERIOSTIN-RELATED; Fasciclin-like protein 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCACACACACCCGCACACACACACACACACACGCAGGTGTGGCTCTGCG
Internal bar code:CGATTTAGACGGGCTGTACATT

Confirmation - LEAP-Seq

LEAP-Seq distance:2019
LEAP-Seq percent confirming:88.1356
LEAP-Seq n confirming:52
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:CACTAGCCACGAAACCTGGT
Suggested primer 2:GCCCTTTGCTCATTACCCCT