| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.044603 |
| Chromosome: | chromosome 10 |
| Location: | 2783137 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g438450 | LTH1 | LAT2 homolog | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTATCTTCTCAGGTGGGATGCAGCACGGCAGTAGCAGCTGGGGCCTCGGC |
| Internal bar code: | CGTCGACAAGTGTTTATATCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1288 |
| LEAP-Seq percent confirming: | 15.0 |
| LEAP-Seq n confirming: | 6 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTCATGCATACACCTTGC |
| Suggested primer 2: | TCGCCTGCTGTTCAACCTAG |