| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.044626 |
| Chromosome: | chromosome 14 |
| Location: | 3587684 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g801659 | CDS | ||
| lncRNA_TCONS_00105207 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACCTTGCGGCCTGGCACAACCGTGTAGCCTAAATAACTTCAAGCTTAGC |
| Internal bar code: | GAGCTTGGGAGCGCGCCCTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 830 |
| LEAP-Seq percent confirming: | 53.8462 |
| LEAP-Seq n confirming: | 7 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCCGTCGGATAGCTTTCTGC |
| Suggested primer 2: | CTGGCAGATTGAAGGGGTGT |