| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044634 |
| Chromosome: | chromosome 3 |
| Location: | 6430081 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g193000 | HAD5 | Halo-acid dehalogenase-like hydrolase; (1 of 1) 3.1.3.29 - N-acylneuraminate-9-phosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTTGAGGGCAGACAGGAAACCGGTTACAGACGTCGTTGTCTTGGCCGT |
| Internal bar code: | CTCATGGCGTCTCTTGGAGCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4488 |
| LEAP-Seq percent confirming: | 93.5897 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTGATCATGCATGGCTG |
| Suggested primer 2: | TGGCGTGAGAAACCGAATCA |