| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.044656 |
| Chromosome: | chromosome 17 |
| Location: | 3941163 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g728000 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCGAAGTGAGTGTCAAGGGCATCCATAGTTGACATGCGACCGTAGCTTA |
| Internal bar code: | ATCGGTTCCCCTTCTAAAATAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1400 |
| LEAP-Seq percent confirming: | 66.6667 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACGCGCCTCATATCCTCAAG |
| Suggested primer 2: | ACGGTTTTGTGTATGCGTGC |