Insertion junction: CLIP2.044658_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.044658
Chromosome:chromosome 6
Location:2533197
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g269400 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCACGCTACTGCGCGCGCGCACACAGACACACGCACACACACACACACAC
Internal bar code:TCTCTAGACGTTATGATTAGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:724
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCAGCTTTATCCGCTTGC
Suggested primer 2:CAAGTGTGAAGTGCCAACCG