Insertion junction: CLIP2.044668_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.044668
Chromosome:chromosome 12
Location:9403035
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g541350 (1 of 14) PF00566 - Rab-GTPase-TBC domain (RabGAP-TBC) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATCCACATCTTGGTGTGCCCCCCCCCTGTTCATGTTCCGCAAGATGGTCT
Internal bar code:TCTTCAACCAAGACAATGCAGT

Confirmation - LEAP-Seq

LEAP-Seq distance:683
LEAP-Seq percent confirming:79.3103
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GCAACCGCAACGATGAATCA
Suggested primer 2:ACACACACACACACGTCCTT