Insertion junction: CLIP2.044681_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.044681
Chromosome:chromosome 9
Location:2501761
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g393000 CSR6 (1 of 6) 2.8.2.8 - [Heparan sulfate]-glucosamine N-sulfotransferase / PAPS:N-desulfoheparin sulfotransferase; Carbohydrate sulfotransferase-related 6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTGTGTGCTCGCATGCCGTGGCGCAGTGACATGCGGCGAGGGAGGTACG
Internal bar code:GCGTGTAATCTGACTAGGTTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:709
LEAP-Seq percent confirming:94.2857
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:ACAAAGGTCGCAAACGTGTG
Suggested primer 2:CTTGCTTGTGCCCATCCCTA