Insertion junction: CLIP2.044685_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.044685
Chromosome:chromosome 6
Location:5438192
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g285850 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGAACGCACGCACACGCACACACACGCACACACGCACACACACGTACG
Internal bar code:TAAGAGCACCATTCGGCTCTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1648
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:6
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:GTCTTCCAGGTCGACATGCA
Suggested primer 2:CACGTGACTGAGAGGTCCAC