Insertion junction: CLIP2.044690_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.044690
Chromosome:chromosome 12
Location:2372554
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g507872 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTCACGATAGCGGCGCCCTGCTGCTCCTGCTCGTCCCAGCTGCCGAAC
Internal bar code:TGTTGTGCGGTCGGTTCTCGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:1704
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGGAGTTTCGCTCATCCT
Suggested primer 2:CAAAGGCATGTCAGCTGCTG