Insertion junction: CLIP2.044706_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.044706
Chromosome:chromosome 1
Location:8076973
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g063632 (1 of 1) K03660 - N-glycosylase/DNA lyase (OGG1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCCACAAGCGCGGGGTCGGAGGTCGCCTGCTGCTGCTGCTGCTGCTGT
Internal bar code:CTGGACTTTACGGGCCTACGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:296
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:TTTTCAACACTCGCAGCTGC
Suggested primer 2:TTTCACCACCGTGTACTCCG