| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044731 |
| Chromosome: | chromosome 2 |
| Location: | 4471864 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g106400 | PDAT1,LCA1 | Phospholipid diacylglycerol acyltransferase; (1 of 1) 2.3.1.158 - Phospholipid:diacylglycerol acyltransferase / PDAT | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAGACCCGAGGTCACAAAGCCTGCACAGCGCGCCAGCAGCAGTCACGGC |
| Internal bar code: | TTCGGGCCTTTTACGCCCAATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1796 |
| LEAP-Seq percent confirming: | 34.4828 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAACACACCGTCCCATAA |
| Suggested primer 2: | ACGGCCTTTCAAGTACCAGG |