| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.044738 |
| Chromosome: | chromosome 9 |
| Location: | 6404924 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g414750 | ELG33 | (1 of 34) 2.4.2.41 - Xylogalacturonan beta-1,3-xylosyltransferase / Xylogalacturonan xylosyltransferase; Exostosin-like glycosyltransferase 33 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCAACGCACAGCCGCGGGTGTCGTTTGCGTAAGGGCAAACTTTCGCTCT |
| Internal bar code: | GCGTGGTTTGTTAACACGGGTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3456 |
| LEAP-Seq percent confirming: | 77.907 |
| LEAP-Seq n confirming: | 67 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 86 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTGCCCGATTAGCGAAGT |
| Suggested primer 2: | AGAGAACACGTTTAGGCCCG |