Insertion junction: CLIP2.044748_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.044748
Chromosome:chromosome 6
Location:5179730
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g283550 CRC2,POC6 Proteome of centriole protein 6; (1 of 1) K16466 - centrin-3 (CETN3, CDC31) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCAGCCGGAAGCAGCTCAAGGTAACTCGGCGCGCCTCCGGGCCGGCCCC
Internal bar code:CAATGGCCCACCCGGTGCGCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:4043
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:GAACCCTTCAAACCGCCAAC
Suggested primer 2:CTCCCTGGAGGTTTCACGTC