Insertion junction: CLIP2.044756_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.044756
Chromosome:chromosome 10
Location:4418504
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g450450 FAP18 Flagellar Associated Protein 18; (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGACATCAACGACAGCCCCGAGGCGCATGCCGAGGTGGACGAGATGGCG
Internal bar code:AGTGGGACTTGGGTTGTTTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1581
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CAGCTTGTAGGTGCCACTGA
Suggested primer 2:GAGGAGGCTGAGGTGATTCG