Insertion junction: CLIP2.044786_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.044786
Chromosome:chromosome 5
Location:3038849
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g231100 (1 of 1) PF00023//PF12796//PF13637 - Ankyrin repeat (Ank) // Ankyrin repeats (3 copies) (Ank_2) // Ankyrin repeats (many copies) (Ank_4) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGAGACAAGGTGAGGCCGCGTGTGGGTGTATTTCGTTGTGTCGAGGAA
Internal bar code:TTAAAGGTTTGCTTCCCCCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:2109
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:GGCACAACACCGAGTACGTA
Suggested primer 2:GACGGATCTGGATTCGGTCC