Insertion junction: CLIP2.044790_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.044790
Chromosome:chromosome 9
Location:1230152
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g400850 CTL4 (1 of 1) PTHR10877//PTHR10877:SF139 - POLYCYSTIN-RELATED // SUBFAMILY NOT NAMED; carbohydrate-binding protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCTGTGGGACACCCGCTGGACCTGGTCCAGTGGGGCCACCTACCGCGAC
Internal bar code:TACTCCTCTGTTTGCTTAAGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:265
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AGAAAGCTCTGCATCCCCAC
Suggested primer 2:GTACAACACCATCGGCTCCA