| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044827 |
| Chromosome: | chromosome 2 |
| Location: | 6921958 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388949 | (1 of 1) PTHR10237 - DEFORMED EPIDERMAL AUTOREGULATORY FACTOR 1 HOMOLOG SUPPRESSIN | CDS | |
| Cre09.g388986 | (1 of 1) PTHR23329//PTHR23329:SF9 - TUFTELIN-INTERACTING PROTEIN 11-RELATED // SUBFAMILY NOT NAMED | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGCAGCGCAGGAGCTTGCCCGGAGCGACGTTGCTACAGGTCGCGCACGC |
| Internal bar code: | ACCGTCTGTAAATGAAAGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3471 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 54 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGCTGAGAAGAGCCAGTT |
| Suggested primer 2: | TAGACAGGGACAGGGACAGG |