Insertion junction: CLIP2.044828_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.044828
Chromosome:chromosome 16
Location:3303598
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g666500 BBS8 (1 of 1) K16781 - tetratricopeptide repeat protein 8 (TTC8, BBS8); Bardet Biedl Syndrome-8 associated protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGACGATCTGGAGATTGAAGAGGAGGTGAGGCGTGCAAGCGGCCCGGG
Internal bar code:TTCCTTTCGACGAAAAATTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:1427
LEAP-Seq percent confirming:89.4737
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:19

Suggested primers for confirmation by PCR

Suggested primer 1:GCCCTGCCCCTTCTTGTAAT
Suggested primer 2:TCAGCCATAAAGAGTGCGCA