| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.044848 |
| Chromosome: | chromosome 16 |
| Location: | 3513386 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g801940 | (1 of 1) PF00078//PF00385//PF00665 - Reverse transcriptase (RNA-dependent DNA polymerase) (RVT_1) // Chromo (CHRromatin Organisation MOdifier) domain (Chromo) // Integrase core domain (rve) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGAACTTCAATTCCGACTTATTGAAGTCGCACTTCGGCAATTTCGCCT |
| Internal bar code: | ATGCCATAACCCGTTACCATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3742 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCAGCTCGCTCGATTTGC |
| Suggested primer 2: | CAAGAGAACAGCTCCCGTGT |