| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044867 |
| Chromosome: | chromosome 12 |
| Location: | 1676235 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g486400 | (1 of 1) PTHR22957//PTHR22957:SF26 - TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEIN // PROTEIN TBC-3, ISOFORM B | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGAGTTGTGTGTGAATGGGTGTGCGTGCTAGGGACACACACGGCCGCCT |
| Internal bar code: | TGGGACGCTACTCGCCGAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1923 |
| LEAP-Seq percent confirming: | 89.4737 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCTCTCAGCTTGTTGAGGA |
| Suggested primer 2: | CCCCGGCACTTCATACTTGT |