Insertion junction: CLIP2.044883_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.044883
Chromosome:chromosome 10
Location:3405253
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g443950 (1 of 2) PF04564//PF05049 - U-box domain (U-box) // Interferon-inducible GTPase (IIGP) (IIGP) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGGGCCAAAGGCGTGCTTCACCGCGGTCACCCCCGGCTTCACCGTTACC
Internal bar code:AACGTGTCAATTCGTATCATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:3493
LEAP-Seq percent confirming:98.1818
LEAP-Seq n confirming:108
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:110

Suggested primers for confirmation by PCR

Suggested primer 1:AACTGCTCTCATGCGAACCA
Suggested primer 2:CAGGGAGCCTTTAGGTGGTG