Insertion junction: CLIP2.044912_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.044912
Chromosome:chromosome 2
Location:1501324
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g084100 (1 of 24) IPR006553 - Leucine-rich repeat, cysteine-containing subtype CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCGACCAGATGGCGGTCAAATTCGAGACCGTGCAGCGCCTCATCCGGCG
Internal bar code:GGGAAAATATTATGTTTTAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2812
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:CCTTACGCACATGCCATTCG
Suggested primer 2:CCTGGACCAGCTGCTTGTTA