| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.044932 |
| Chromosome: | chromosome 10 |
| Location: | 3344328 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g443450 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCAATCCTTCATTTTGCTCGTGCTGTCCGCCAAGAGGCAACTGGACTGTC |
| Internal bar code: | ATATTGGAGACGAGGTCACCTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 802 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCACAAATAAGCGACGCCG |
| Suggested primer 2: | GTACATGTCCGCGATCGTCT |