Insertion junction: CLIP2.044953_3

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.044953
Chromosome:chromosome 4
Location:2343989
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g221000 (1 of 3) PTHR23024//PTHR23024:SF145 - MEMBER OF 'GDXG' FAMILY OF LIPOLYTIC ENZYMES // ISOPRENYLCYSTEINE ALPHA-CARBONYL METHYLESTERASE ICME-RELATED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGCAAGCACCACAACCACCACCACAACCACAACCACGACCACAACCACC
Internal bar code:TTGATTATCCCACGAGGTCCAG

Confirmation - LEAP-Seq

LEAP-Seq distance:2697
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:70
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:70

Suggested primers for confirmation by PCR

Suggested primer 1:CACGCACAGCTTCAGCATTT
Suggested primer 2:GAGCTGTTGGGAGATGCCAT