Insertion junction: CLIP2.044963_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.044963
Chromosome:chromosome 17
Location:1863918
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g709500 (1 of 52) 2.7.11.22 - Cyclin-dependent kinase / Cdk-activating protein kinase 5'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTTAATGCCCCGTACCCTGTCCCATCGCGCCCTGCACACACATAGAAAGC
Internal bar code:TGATCGCAAGAATTTACGCTCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1788
LEAP-Seq percent confirming:92.0
LEAP-Seq n confirming:23
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:TTTCAGTTGCGTTGCCGAAG
Suggested primer 2:TAGGCCAATGGATGCTTGCA