| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.044968 |
| Chromosome: | chromosome 11 |
| Location: | 1167364 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467680 | (1 of 13) 3.1.13.4 - Poly(A)-specific ribonuclease | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGAGGGGTGTGGCACAGAATGGCTGCAGTCGCAGAAATAAAAGGGCAC |
| Internal bar code: | AGGGATGCACTGTGGTGGATAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1395 |
| LEAP-Seq percent confirming: | 55.0 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTTGACACTCTGCTGCAGA |
| Suggested primer 2: | TTCACCTCCATCCACAACGG |