| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044978 |
| Chromosome: | chromosome 2 |
| Location: | 1449264 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g083650 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCCACCTGTGGGCAGGGTGGCCGCTTAGCAACGTCTGGCGCTGACAATG |
| Internal bar code: | TGTAGCTAGGGATCGAACTCCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3074 |
| LEAP-Seq percent confirming: | 90.1961 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGTCTGAGGGTTGTCTCGC |
| Suggested primer 2: | CCGATGGAGAAGCTGTTGGT |