| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.044980 |
| Chromosome: | chromosome 8 |
| Location: | 3391705 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g377900 | (1 of 7) PF05869 - DNA N-6-adenine-methyltransferase (Dam) (Dam) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTCGAGGCATGCCGAGTCAACAGGGTGCATGCATGTGTAGAGGATGGG |
| Internal bar code: | TAGGGTGAAACTAGGATATACA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1380 |
| LEAP-Seq percent confirming: | 47.1264 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 46 |
| LEAP-Seq n unique pos: | 87 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTTCTCCTGCTCCTCCT |
| Suggested primer 2: | ACGTACACAACCACCATCCC |