| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.044982 |
| Chromosome: | chromosome 13 |
| Location: | 946552 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g568100 | (1 of 4) 3.4.21.19 - Glutamyl endopeptidase / V8 proteinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGTTTACGGATGTTACCTGAAGTTACCTCGCACACCTGCTGCGTTGAGC |
| Internal bar code: | GTCTGCTTATTAGGACGGGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1011 |
| LEAP-Seq percent confirming: | 16.6667 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCGCTAACATGTTCCTTCG |
| Suggested primer 2: | CGTCCTCTTTCCCAGGTGTC |