| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.044993 |
| Chromosome: | chromosome 6 |
| Location: | 5118403 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282900 | (1 of 1) K09288 - coiled-coil domain-containing protein 6 (CCDC6, PTC) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCAAAGCAGACCATCCGGACGATGCAGTGCGGCTTCCTACAAGCGTGGA |
| Internal bar code: | CTCACTTTGATATGTTGGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1841 |
| LEAP-Seq percent confirming: | 86.9565 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGACCAACTCAAACGGGAG |
| Suggested primer 2: | TTCTGCAGCCGGTTCAGAAT |