Insertion junction: CLIP2.044999_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.044999
Chromosome:chromosome 5
Location:2311940
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g238260 (1 of 1) PF13371//PF13414//PF13432 - Tetratricopeptide repeat (TPR_9) // TPR repeat (TPR_11) // Tetratricopeptide repeat (TPR_16) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAACCTTGTAACCCCACCCCACCCCACCCTACTCCTTCACTTCGTAAACA
Internal bar code:GGGGGGTAAGCGATAATGTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:115
LEAP-Seq percent confirming:87.5
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:GACCAGACAGGGGGAAGTTG
Suggested primer 2:GGCCGTACGTTCATGATTGC