| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.045035 |
| Chromosome: | chromosome 1 |
| Location: | 2900685 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g018050 | NEF1 | (1 of 1) PTHR35313//PTHR35313:SF1 - FAMILY NOT NAMED // NO EXINE FORMATION 1; NEF1-like protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAGCGGCCTGGCGCTGCTGTTGCACGCCGAGCTGCCGGTGTCGGCGGAC |
| Internal bar code: | CATGGAGTAAGTGTGACCGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3948 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 59 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 59 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGCAGCGAGTTGGATTGA |
| Suggested primer 2: | CGAAGACGTGGAGATCTGGG |