Insertion junction: CLIP2.045053_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045053
Chromosome:chromosome 1
Location:3971386
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g025983 protein of unknown function with DUF1399 domain; (1 of 11) PF07173 - Protein of unknown function (DUF1399) (DUF1399) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACAAACAGACACCCGCCCGGCGCCTGCGCCTGCAACCCCTGCGTTCGACG
Internal bar code:CATGCCTGGACCAAGCTCGGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:155
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:10
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:TACATCGCGCTCTGGACATC
Suggested primer 2:CACAAGAGGCGGATCCACTT