Insertion junction: CLIP2.045059_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.045059
Chromosome:chromosome 4
Location:3386692
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g227500 SRR18 Scavenger receptor cysteine rich (SRCR) protein; (1 of 2) IPR001190//IPR006626//IPR011050//IPR017448 - SRCR domain // Parallel beta-helix repeat // Pectin lyase fold/virulence factor // SRCR-like domain CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTTGAATCCAACTCGGCCGGGCAGGCGGGCGGCGGCGCTGCGGTGCTG
Internal bar code:TTCCGAGTAGAGTGGCGTTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:690
LEAP-Seq percent confirming:75.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGCTGTCGTCATTCATG
Suggested primer 2:TGTTCCTGGCAACACTGGTT