Insertion junction: CLIP2.045062_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.045062
Chromosome:chromosome 2
Location:7892132
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g145700 AOT1 Amino acid transporter; (1 of 2) K14207 - solute carrier family 38 (sodium-coupled neutral amino acid transporter), member 2 (SLC38A2, SNAT2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCTCTCCCACCTTTGTGACGCTCTCTCGCACGGCATCTCCCACACGCT
Internal bar code:GCACGGTTCTTCGTCGCTTGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:3198
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:40
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:40

Suggested primers for confirmation by PCR

Suggested primer 1:GCAATACACATGGCGCGAAT
Suggested primer 2:CAGTGCGTGTGATGGCAAAA