Insertion junction: CLIP2.045079_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.045079
Chromosome:chromosome 2
Location:833005
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g078777 PHC16 Putative pherophorin-chlamydomonas homolog; (1 of 71) PF12499 - Pherophorin (DUF3707) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGAAGTTGACGAGCTGTTCCAGCACGTCGCCCACCTCGGGCTGGAACAG
Internal bar code:TTCCAGGTTCATTGGGTGGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1414
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:37
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:37

Suggested primers for confirmation by PCR

Suggested primer 1:CACTTGCGCATACATGGTCG
Suggested primer 2:GTCAGCGAGGGGAACTTCAA