| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.045082 |
| Chromosome: | chromosome 1 |
| Location: | 4170689 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g027900 | (1 of 1) K17338 - receptor expression-enhancing protein 1/2/3/4 (REEP1_2_3_4); similar to HAV22 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGAGGTGTATGTGACGGGACAACTGGCGGTGAGGGCGGGAGACGGGCAC |
| Internal bar code: | TATTGCGCTCGAGGATTGGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3433 |
| LEAP-Seq percent confirming: | 75.0 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 120 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCACGAACAACAGCTTGG |
| Suggested primer 2: | GTTCCCAGCCTACATGACCC |