| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.045090 |
| Chromosome: | chromosome 16 |
| Location: | 5844593 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g679800 | (1 of 2) PTHR17630//PTHR17630:SF43 - DIENELACTONE HYDROLASE // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCCCCTCTCCAACCCAATGACATCTTTGACTCGTATAGTCATATGGCG |
| Internal bar code: | ATAATTATCTACGAATTAAGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1273 |
| LEAP-Seq percent confirming: | 69.3878 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 49 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGTGAGGGGTCCATGCTTG |
| Suggested primer 2: | CATCAATCTGCGGCTTGAGC |