Insertion junction: CLIP2.045107_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.045107
Chromosome:chromosome 14
Location:1963687
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g801624 (1 of 7) 2.7.10.2//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Dual-specificity kinase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCGCAACCGCGGTGGCGGCGGCCGCCGAGACGCTGGTCGCCGTCGCTGC
Internal bar code:TTGCTCCGTCGAGAGGGCTTGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1898
LEAP-Seq percent confirming:97.1429
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:35

Suggested primers for confirmation by PCR

Suggested primer 1:GACTGAACACGGTGTCGGAA
Suggested primer 2:AGTACATGGTAGCGCTGTCG